More on the fructosamine assay
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چکیده
منابع مشابه
Influence of timing in the fructosamine assay.
The fructosamine assay, based on the measurement of the reducing activity in serum at alkaline pH, provides an index of protein glycation. The reducing activity is expressed in equivalents of 1-deoxy-1-morpholinofructose (DMF) by direct comparison with the activity either of this synthetic compound or with a secondary protein standard calibrated against DMF. This study reports the influence of ...
متن کاملEffects of albumin and immunoglobulin A on fructosamine assay.
Serum fructosamine, albumin, and IgA were measured in three groups of human subjects: 54 nondiabetic normal individuals, 149 nondiabetic patients, and 149 diabetic patients. Normal subjects had significantly (P less than 0.05) higher fructosamine (2.91, SD 0.33, mmol/L) than did nondiabetic patients (2.49, SD 0.46, mmol/L). Each of these groups had significantly (P less than 0.05) lower fructos...
متن کاملInhibitory effect of superoxide dismutase on fructosamine assay.
The fructosamine assay measures the degree of nonenzymatic protein glycation by virtue of the reducing properties of such proteins in alkaline conditions. We report the marked inhibitory effect of superoxide dismutase (EC 1.15.1.1) on the reducing activity both of protein glycated in vitro and of diabetic sera, indicating superoxide intermediacy in the fructosamine reaction. The free-radical in...
متن کاملFructosamine assay using albumin extracted from serum.
Albumin extracted from serum by a simple technique using trichloroacetic acid and ethanol has been applied to a fructosamine assay using nitroblue tetrazolium. A fructosamine assay using extracted albumin sample was carried out without interference from low molecular weight substances with reducing activities and other proteins with varying concentrations, half-lives and reducing activities. 1-...
متن کاملFructosamine assay modified for the estimation of glycated hemoglobin.
CLINICAL CHEMISTRY, Vol. 35, No. 3, 1989 497 4#{176}C. For 12 additional patients (group B) the analysis was performed immediately and repeated 10 and 30 days after collection of the samples, which were stored at room temperature in airtight containers. The data were examined by Student’s paired t-test and linear-regression analysis. In neither group did storage conditions significantly influen...
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ژورنال
عنوان ژورنال: Diabetologia
سال: 1987
ISSN: 0012-186X,1432-0428
DOI: 10.1007/bf00295887